63.5%GC3 content

52.1Effective codons (Nc)

250Coding sequences

4/26GC3 rank here

Scope: nuclear genes only

NCBI table 1 (Standard). Do not apply these frequencies to mitochondrial genes, where AGA, AGG, ATA and TGA carry different meanings. The four reassignments.

What distinguishes this table

The most constrained choice in Green anole (lizard) is glutamine: of its 2 synonymous codons, CAG takes 72% of the family. Phenylalanine sits at the other end, spread almost evenly across its options (evenness 0.99 against 0.85 for glutamine). Those two families are where a codon-optimizer has the most and the least room to move.

Measured across all 59 sense codons, this table correlates most closely with Rhesus macaque (r = 0.982) and least with Ferret (r = 0.744). A sequence tuned for the first would need little adjustment to move here; one tuned for the second would need a great deal.

Relative to the average across the 26 organisms on this site, Green anole (lizard) leans hardest on TGC (+0.147 against the mean) and avoids TGT most (-0.147). Those two are the codons that most distinguish this table from the others.

Preferred and avoided codons

Amino acid	Preferred	Freq	RSCU	Avoided	Freq	RSCU
Alanine A	GCC	0.480	1.92	GCG	0.138	0.55
Arginine R	CGG	0.272	1.63	CGT	0.058	0.35
Asparagine N	AAC	0.639	1.28	AAT	0.361	0.72
Aspartate D	GAC	0.598	1.20	GAT	0.402	0.80
Cysteine C	TGC	0.687	1.37	TGT	0.313	0.63
Glutamate E	GAG	0.576	1.15	GAA	0.424	0.85
Glutamine Q	CAG	0.722	1.44	CAA	0.279	0.56
Glycine G	GGC	0.352	1.41	GGT	0.135	0.54
Histidine H	CAC	0.659	1.32	CAT	0.341	0.68
Isoleucine I	ATC	0.535	1.61	ATA	0.121	0.36
Leucine L	CTG	0.349	2.10	TTA	0.053	0.32
Lysine K	AAG	0.595	1.19	AAA	0.405	0.81
Phenylalanine F	TTC	0.558	1.12	TTT	0.442	0.88
Proline P	CCC	0.339	1.35	CCG	0.199	0.80
Serine S	TCC	0.276	1.66	TCA	0.086	0.51
Threonine T	ACC	0.419	1.68	ACG	0.170	0.68
Tyrosine Y	TAC	0.604	1.21	TAT	0.396	0.79
Valine V	GTG	0.428	1.71	GTA	0.072	0.29

Full codon usage table

Amino acid	Codon	Frequency	RSCU
Alanine A	GCC	0.4803	1.921
	GCT	0.2005	0.802
	GCA	0.1809	0.724
	GCG	0.1383	0.553
Arginine R	CGG	0.2716	1.630
	CGC	0.2291	1.375
	AGG	0.1839	1.103
	AGA	0.1594	0.956
	CGA	0.0980	0.588
	CGT	0.0579	0.347
Asparagine N	AAC	0.6394	1.279
Asparagine N	AAT	0.3606	0.721
Aspartate D	GAC	0.5984	1.197
Aspartate D	GAT	0.4016	0.803
Cysteine C	TGC	0.6867	1.373
Cysteine C	TGT	0.3133	0.627
Glutamate E	GAG	0.5762	1.152
Glutamate E	GAA	0.4238	0.848
Glutamine Q	CAG	0.7215	1.443
Glutamine Q	CAA	0.2785	0.557
Glycine G	GGC	0.3517	1.407
	GGG	0.2754	1.102
	GGA	0.2378	0.951
	GGT	0.1351	0.540
Histidine H	CAC	0.6593	1.319
Histidine H	CAT	0.3407	0.681
Isoleucine I	ATC	0.5353	1.606
	ATT	0.3436	1.031
	ATA	0.1211	0.363
Leucine L	CTG	0.3495	2.097
	CTC	0.2695	1.617
	TTG	0.1561	0.937
	CTT	0.1130	0.678
	CTA	0.0585	0.351
	TTA	0.0535	0.321
Lysine K	AAG	0.5947	1.189
Lysine K	AAA	0.4053	0.811
Methionine M	ATG	1.0000	1.000
Phenylalanine F	TTC	0.5585	1.117
Phenylalanine F	TTT	0.4415	0.883
Proline P	CCC	0.3386	1.354
	CCT	0.2328	0.931
	CCA	0.2298	0.919
	CCG	0.1989	0.796
Serine S	TCC	0.2764	1.658
	AGC	0.2755	1.653
	TCT	0.1481	0.889
	TCG	0.1107	0.664
	AGT	0.1036	0.622
	TCA	0.0857	0.514
Threonine T	ACC	0.4189	1.676
	ACA	0.2197	0.879
	ACT	0.1910	0.764
	ACG	0.1704	0.682
Tryptophan W	TGG	1.0000	1.000
Tyrosine Y	TAC	0.6042	1.208
Tyrosine Y	TAT	0.3958	0.792
Valine V	GTG	0.4281	1.712
	GTC	0.3304	1.322
	GTT	0.1691	0.676
	GTA	0.0724	0.290

Provenance

Computed from 250 RefSeq coding sequences for Anolis carolinensis, sampled and counted codon by codon. Frequencies are relative within each amino acid family. Page generated 2026-08-09.

The full derivation of GC3, RSCU and the effective number of codons, along with the limits of each, is set out once in the methods note rather than repeated on every page.

Use this table

Nucleora codon-optimizes a coding sequence against this table directly, then folds the result and reports where structure would interfere with expression.

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